DISCOVERY OF A NOVEL AND ECOFRIENDLY BIOPESTICIDES DERIVED FROM WILD TYPE AND TRANSGENIC BRASSICA JUNCEA CALLUS MODULATED WITH GAMMA-TOCOPHEROL METHYLTRANSFERASE GENE FOR SUSTAINABLE AGRICULTURE
Adetunji Charles Oluwaseuna,b, Paomipem Phazangb, Adetunji Juliana Bunmic, Neera Bhalla Sarinb,
aEdo University, Department of Microbiology, Applied Microbiology, Biotechnology and Nanotechnology Laboratory, Iyamho, Edo State. Nigeria.
bJawaharlal Nehru University, School of Life Sciences, Laboratory of Genetic Manipulation for Stress Alleviation and Value Addition in Plants, New Delhi-110067. INDIA
c Osun State University, Nutritional and toxicological Research Laboratory, Department of Biochemistry, Osogbo Nigeria.
This study utilized secondary metabolites produced from the wild Brassica juncea callus (PMWC) modulated with gamma TMT gene for the production of ecofriendly biopesticides with high tolerance to abiotic and biotic stress in a tomato plant. The phytotoxic metabolites from the transgenic callus (PMTC) were produced from the Agrobacterium-mediated transformation of the wild callus of Brassica juncea.
Pest and pathogens have been highlighted as a major constrains affecting the increase in agricultural productivity globally. The persistent use of pesticides has led not only to pest and disease resistance but also, bioaccumulation, biomagnification which subsequently affect human health and consequently result to environmental pollution.
The in-vitro biological control efficacy of these phytotoxic metabolites was evaluated in a greenhouse in comparison with synthetic fungicide. The physiochemical and antioxidant properties were evaluated on the harvested tomato fruits while the in-vivo biochemical assessment of the harvested tomato fruits was carried-out using an albino rat.
The greenhouse evaluations showed a significant increase (p>0.05) in biological control efficacy, growth and yield components of the tomato plant treated with PMTC followed by PMWC when compared with the synthetic fungicide. The harvested tomato fruits showed a reduction in weight loss, ascorbic acid value, soluble solids content, enhanced firmness, higher lycopene content, increase in the activity of catalase, peroxidase, superoxide dismutase and prevented the release of superoxide free radicals in the following order PMTC > PMWC > synthetic fungicide. The histopathological and biochemical analysis on albino-rat fed with harvested tomato fruits shows mild toxicity with PMTC followed by PMWC when compared to a synthetic fungicide.
Finally, considering the broad-spectrum antifungal activity, plant growth-supporting characteristic, antioxidant promoting qualities, and human health friendliness, make these biopesticides particularly attractive for efficient agricultural applications in plant disease management.