Arwa Elsamany posteradmin
GENETIC POLYMORPHISM AT LEPTIN GENE IN HOLSTEIN FRIESIAN Bos taurus X KENANA Bos indicus CROSSBREED CATTLE IN SUDAN
Arwa Alsammany1 , Sumaia M.A. Abukashawa1
1Faculty of Science, University of Khartoum
Background: The importance of reproduction traits in dairy cattle breeding programs has dramatically increased recently. Leptin gene is one of the candidate genes, which located at chromosome 4 and is consisted from 3 exons and 2 introns. Identification of single nucleotide polymorphisms (SNP) opened new vistas in animal breeding and several polymorphic studies on the bovine leptin gene have been reported, SNP LEP/Sau3AI is situated in the second intron. Objective: The objective of this research was to evaluate the frequency of leptin gene polymorphism among Bos indicus and Bos Taurus crossbred cattle in Sudan. Materials and Methods: This study was cross sectional study. During 2016 blood was collected from Holstein Friesian x Kenana crossbreed into EDTA sterile tubes, AA, AB, BB genotypes were detected using PCRRFLP technique. Data analysis was done by using SPSS software. Finding: Genotype AA was found to be frequent in LEP/Sau3AI SNP. Conclusion: Genotype AA is more frequent in LEP/Sau3AI SNP, suggesting that it can be used as a good genetic marker in selection and breeding of dairy cattle.