Amna Omer posteradmin
MORPHOLOGICAL AND MOLECULAR IDENTIFICATION OF BULINUS SPECIES FROM KHARTOUM, GEZIRA AND WHITE NILE STATES USING CYTOCHROME OXIDASE SUBUNIT1 (CO1)
Miss. Amna Osman Ali Mobarak1,
Dr. Mushal Allam2,
Prof. Ahmed Babiker Mohammed Ahmed3,
Dr. Mubarak Mustafa Abdelrahman1,
- Tropical Medicine Research Institute (TMRI).
- National Institute for Communicable Diseases, Johannesburg, South Africa.
- Ahfad University for woman.
The current study Aim to identify Bulinus species the intermediate host of Schistosoma haematobium. A total of 1203 Bulinus spp. Snail were collected from three states in Sudan; Khartoum, Gezira and White Nile States. Morphometric parameters measured using (Imagej) program. The Result showed that 32% were B. forskalii and 78% belong to B. truncatus, however the limitations of the morphological methods prompted us to amplify and sequence a 700 bp fragment of the mitochondrial gene cytochrome oxidase subunit 1 (cox1) for further subtyping of the two species snails characterized phenotypically. Haplotype and phylogeny analyses of the sequenced cox1 gene characterized further two haplotypes in the B. forskalii and four haplotypes in the B. truncatus. The results reported in this study are very important since a detailed understanding of the population genetic structure of Bulinus spp is essential to understand the epidemiology of many schistosome parasites.