Frequencies of BCR-ABL1 fusion transcripts among Sudanese chronic myeloid leukaemia patients
Emad-Aldin Ibrahim Osman, Faculty of Medical Lab Sciences, Elrazi University
Kamal Hamad, Faculty of |Medicine, University of Khartoum
Imad Fadl Elmula Al Nelein University
Muntaser Eltyeb Ibrahim Institute of Endemic Diseases, University of Khartoum
The incidence of one or other rearrangement in chronic myeloid leukemia (CML) patients varies in different reported series.
In this study we report the frequencies of BCR-ABL1 fusion transcript variants studied in 43 CML patients from Sudan using Reverse transcriptase-Polymerse Chain Reaction (RT-PCR). The study includes 46 Sudanese patients.
Out of the 46 patients, three of which negative for the BCR-ABL1 fusion transcript. More than half of 43 positive patients showed b2a2 fusion transcript (53.5%), while (41.9%) showed b3a2 transcript and the remaining (4.6%) coexpression of b3a2/ b2a2 and b3a2/b2a2/e19a2. We detected neither coexpression of p210/p190 nor e1a2 alone. Male patients showed a tendency to express b2a2, while female tende to express b3a2 (p = 0.017). Moreover, a single nucleotide polymorphism was detected in BCR exon 13 in one out of four patients and this patient showed only b2a2 expression.
In conclusion, we observed a significant correlation between sex and type of BCR-ABL1 transcript, an observation that deserves further investigation.
Dr. Emad Aldin Ibrahim Osman Ramadan
Faculty of Medical Laboratory Sciences, Elrazi University, Khartoum, Sudan