Hala Abdalazeem

ASSOCIATION OF BODY MASS INDEX AND OBESITY RELATED FTO GENE (RS1421085), ( RS17817449)AND (RS9939609) POLYMORPHISMS IN OBESE ADULTS , GEZIRA STATE, SUDAN (2017)

Hala A. Abdalbasit 1, Taiseer M. Ibrahim 2, Eman M. Yousif 3, Amear M. Dafallah4 , Hisham N. Altyeb5, Adam D. Abakar6

1Department of Clinical Chemistry, Faculty of Medical Laboratory Science University of Gezira Wadmedani, Sudan (presenter)

2Faculty of Medical Laboratory Science, Omdurman Islamic University, Khartoum, Sudan

3Faculty of Medical Laboratory Science, University of Gezira, Wadmedani, Sudan

4National Cancer Institute, University of Gezira, Wadmedani, Sudan

5Department of Microbiology, Faculty of Medical Laboratory Sciences, Sudan University of Science and Technology, Khartoum, Sudan

6Deanship of scientific research University of Gezira, Wadmedani, Sudan

Abstract

This case control study aimed to assess the association of the FTO SNPs rs9939609, rs17817449 and rs1421085 with dietary intake, eating behavior and physical activity and secondly to determine the effect of the interaction between these FTO polymorphisms and the mentioned variables on BMI in the Sudanese adults. A cluster of single nucleotide polymorphisms in intron one of FTO gene have been associated with body mass index (BMI) and obesity. Sixty obese Sudanese adults were participated, BMI was calculated and structured questionnaire was used to asses food intake, eating behavior and physical activities. Guanidine Chloride method was used for DNA extraction, then PCR was carried to amplify FTO SNPs rs9939609, rs17817449 and rs1421085 region, normal sequencing was used to detect the SNP and the sequence was analyzed using Finsh T.V and Bio Edit programs. The presence of FTO rs9939609 and rs17817449 polymorphisms was detected and there was no rs1421085 within FTO gene in Sudanese obese adults. Another new polymorphism FTO rs9926289 and rs8046757 was detected when analyzing FTO rs9939609 and rs17817449 respectively. Novel FTO8063946 was found in one case which needs further study to detect it is effect. When comparing BMI and the main diet, there was significant statistical association (p. value =0.02) while with physical activity there was no association (p value =0.08).The sample size restricted statistical power of analyses, so further studies should be done to confirm the reliability.

 

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