Hana Elbadawi Posteradmin
DETECTION AND MOLECULAR CHARACTERIZATION OF CARBAPENEMASE PRODUCING GRAM NEGATIVE BACILLI AMONG HOSPITALISED PATIENTS IN KHARTOUM STATE
Hana Elbadawi1, 2, Kamal Elhag1, 3, Elsheikh Mahgoup4, Muzamil Mahdi Abdel Hamid2
1Soba University Hospital, Khartoum, Sudan
2Institute of Endemic Diseases, University of Khartoum, Khartoum, Sudan
3Ahfad University for Women, Omdurman
4Department of Microbiology, Faculty of Medicine, University of Khartoum, Khartoum, Sudan
Carbapenems are broad-spectrum antibiotics widely used as the last-line antibiotic for treatment of multi drug resistant Gram-negative bacilli isolates in systemic infection. In past ten year the Carbapenem resistance among gram-negative bacilli has been rapidly growing across nosocomial infection isolates.
This study aimed at evaluating the prevalence and molecular characterization of Carbapenem resistance genes among Gram-negative bacilli (GNB) isolated from patients treated in hospitals at Khartoum state, Sudan.
Across-sectional laboratory based study was conducted over six month period at the microbiology department in Soba University Hospital and Institute of Endemic Diseased, University of Khartoum. A total of 734 GNB isolates from different clinical specimen were analyzed for carbapenem resistance genes using phenotypic test and confirmed by gene detection. Multiplex PCR was performed for each isolate to detect the major carbapenemase genes, including those encoding the NDM-, VIM-, and IMP-type metallo-beta-lactamases, the class A KPC-type carbapenemases, and the class D OXA-48 enzyme. In addition to CTXM, TEM and SHV.
Of 734 isolates, 171 (23.2%) positive phenotypically and 121 (70.7%) from 171 isolates were positive for one or more carbapenemase gene. NDM-types were the most predominant genes in particular blaNDM-1 107(88.4%), followed by blaIMP 7 (5.7%), blaOXA-48 5(4.1%), blaVIM 2 (1.6%) and blaKPC 0 (0%). Co resistance genes with NDM producer Gram-negative bacilli were detected in107NDM positive isolates, NDM+ CTXM in 50 (46.7%), NDM+ TEM 44 (41.1%), and NDM+ SHV 40 (37.3%). Carbapenemase genes were mostly detected in the K. pneumoniae 64 (52.8%), followed by P. aeruginosa 19 (15.7%), A. baumannii 18 (14.8%) and E. coli with 12 isolates (9.9%).
There was high prevalence of carbapenemase producing bacilli in our setting, that we need for implementation of strict infection control measures and regular surveillance to prevent further spread of these resistant organisms among the hospital isolates.
Poster presenter Hana Salaheldin Elbadawi, BSc, MSc, PhD student.
Soba University Hospital, Khartoum, Sudan
Institute of Endemic Diseases, University of Khartoum, Khartoum, Sudan