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MOLECULAR TRACING OF HEPATITIS C VIRUS GENOTYPE 1 ISOLATES IN NIGERIA: 2000-2018
Awoyelu E. H.1, Oladipo E.K.2, Oloke J.K.1
1 Department of Pure and Applied Biology (Microbiology / Virology Unit), Ladoke Akintola University of Technology, Ogbomoso, Oyo State, Nigeria
2 Department of Microbiology, Molecular Biology, Immunology and Bioinformatics Laboratory, Adeleke University, Ede, Osun State, Nigeria.
Background: Hepatitis C virus (HCV) has been classified into 7 genotypes and different subtypes. This classification is distributed through various risk groups and origins in geographical regions. Hence, a well-established phylogenetic relationship can simplify the tracing of HCV hierarchical strata into geographic regions.
Aim and Objectives: This study aimed at investigating the genetic relationship among all HCV-1 sequences derived from Nigeria and some selected countries by applying phylogenetic analysis based on NS5B nucleotide sequences with an understanding of the source of spread of HCV-1 in Nigeria.
Methods: Phylogenetic analysis was performed based on nucleotide sequences of NS5B gene of HCV-1 which were registered in GenBank within the years 2000 and 2018.
Results: In this study, 25 sequences comprising of 5 sequences from Nigeria, 5 from Ghana, 5 HCV-1 sequences from Iran, 5 HCV-1 sequences from USA, 5 HCV-1 sequences from United Kingdom underwent phylogenetic analysis. Phylogenetic analysis of NS5B gene from all the sequences revealed two lineages with different clusters in the phylogenetic tree. It also showed the likelihood of HCV-1 isolates from Nigerian patients having similarities with Ghanaian patients.
Conclusions: The obtained phylogenetic tree of the analyzed HCV-1 sequences from this study has provided the ancestral relationship of the HCV-1 isolates from Nigerian patients with Ghanaian patients, and also showed the likelihood of domestic origin as the source of spread.
Correspondence: Awoyelu E.H., Department of Pure & Applied Biology (Microbiology/Virology Unit), Ladoke Akintola University of Technology, P.M.B. 4000, Ogbomoso, Nigeria, firstname.lastname@example.org