TRIB-1 & MIR-202 EXPRESSION UNDER METABOLIC AND INFLAMMATORY STRESS CONDITIONS IN HEPG2.
*1Phillip, I. O. and 1Phillip, J. O
1Department of Genetics and Biotechnology, University of Calabar, PMB 1115, Calabar, Nigeria
*Author for correspondence: firstname.lastname@example.org ; +2348067880200
Aim: To evaluate the endogenous expression of MIR-202 in an hepatic cell line and its corresponding effects on trib-1 level under conditions of metabolic and inflammatory stress.
Background: The Cardiovascular disease burden is a global one which accounts for high rate of mortality and morbidity in both developed and developing countries and as such, the development of new therapeutics targeting its onset is unending. Research from genome wide association studies (GWAS) has implicated human polymorphisms of the trib-1 gene to be associated with increased risk factors for cardiovascular diseases (CVD). MicroRNA studies have shown that trib-1 transcript is a target for MIR-202 and as such could have an effect on its protein stability.
Methods: HepG2 cells were cultured under in vitro conditions of high glucose and cytokine stimulation with interleukin-1 under concentrations of varying time intervals of 24hrs & 48hrs, after which the cells were harvested and mRNA of trib-1 and MIR-202 extracted using the spin-colum-based centrifugation, reversed transcribed and analysed for endogenous expressions of both trib-1 and MIR-202 using qPCR.
Result: it was observed that there was a significant (p < 0.05) decrease in trib-1 levels under these treatments of high glucose, stimulation with interleukin-1 and also a combination of both treatments whilst there was a consistent pattern of upregulation of MIR-202 in this conditions.
Conclusion: These suggests a possible interaction between trib-1 and MIR-202 which could affect trib-1 stability and also the potentials for MIR-202 to be involved in some cellular activities in HepG2 cells relating to this conditions and is in agreement with previous research findings which reveals the instability of trib-1 in lipid metabolism and could serve as a unique biomarker for such conditions and metabolic activities taking place in the liver.
Mrs Iquo Oyohosuho Phillip
Department of Genetics & Biotechnology
Faculty of Biological Science
University of Calabar,