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TITLE : EXPLORATION OF THE ANTIBACTERIAL AND CHEMICAL POTENTIAL OF SOME BENINESE PHARMACOPOIEA TRADITIONAL PLANTS
Boris Lègba,1 Victorien Dougnon, 2 Kafayath Fabiyi,2 Jerrold Agbankpè,2 Gildas Hounmanou,2 Alidah Aniambossou,2 Edna Hounsa,2 Affousssath Amadou,2 Phénix Assogba,2 Honoré Bankolé,2 Jacques Dougnon,2 Lamine Baba-Moussa1
1Laboratory in Biology and Molecular Typing in Microbiology, Faculty of Sciences and Techniques, 2Research Unit in Applied Microbiology and Pharmacology of Natural Substances, Research Laboratory in Applied Biology, Polytechnic School of Abomey-Calavi, University of Abomey-Calavi, Cotonou, Benin
Aim: This study aims to evaluate the antibacterial and chemical properties of some medicinal plants used in the fight against enteropathogens in Benin.
Background: Infections caused by enteropathogens are serious forms of infectious pathology. They are a major public health problem that causes millions of deaths a year.
Methods : Aqueous and ethanolic extracts of Senna siamea, Uvaria chamae, Lantana camara and Phyllantus amarus were tested on 10 bacterial strains. Well diffusion technique, coupled with the microdilution determination of Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (CMB) was used for antibacterial testing. The larval cytotoxicity was evaluated by using Artemia salina crustacean larvae. flavonoids and polyphenols were also assayed by the method using aluminum trichloride (AlCl3) and the method using the folin-Ciocalteu reagent, respectively.
Results : The results of the study revealed that extracts had an effective antibacterial activity at 100 mg/mL, with MIC between 100 and 25 mg/mL and CMB between 100 and 50 mg/mL. The inhibition diameters of the extracts varied between 7.5 and 21 mm. The ethanolic extract of Phyllantus amarus leaves showed the best antibacterial activity. None of the extracts tested was found to be cytotoxic at the dose of 20 mg/mL. The aqueous Uvaria chamae root extract has the highest polyphenol content (231.896552±0.27586207 in μg EAG/100 mg extract), whereas the aqueous leaf extract of Uvaria chamae is the richest in flavonoids (41.061082 0.43180737 in μg ER/100 mg of extract).
Conclusions: These interesting results can be used in the development of improved traditional medicines against enteropathogens.