COMPARATIVE GENOMIC AND PHYLOGENETIC ANALYSIS OF ZIKA VIRUS FROM NIGERIA, BRAZIL AND MEXICO
*1,2Oladipo E.K., 2Awoyelu E. H., 1Oyawoye O.M., 1Odedeji O.D., 3Oluremi A.S.,
1 Department of Microbiology, Laboratory of Molecular Biology and Bioinformatics, Adeleke University, Ede, Osun State, Nigeria.
2 Department of Pure and Applied Biology (Microbiology / Virology Unit), Ladoke Akintola University of Technology, Ogbomoso, Oyo State, Nigeria.
3 Department of Medical Laboratory Sciences, College of Health Sciences, Ladoke Akintola University of Technology, Ogbomoso, Oyo State, Nigeria.
Aim: In this study, a comparative genomic and phylogenetic analysis was performed on the selected genome sequences of Zika virus polyprotein from Nigeria, Brazil and Mexico available in the NCBI Nucleotide database.
Background: Epidemiological data have suggested the presence of Zika virus in some countries. Zika virus infections have been reported in Nigeria in 1979 with new outbreaks in Brazil and Mexico. However, the genomic and phylogenetic analysis of the strains that circulate in the selected countries is of great epidemiological importance.
Methods: The polyprotein genome of the Zika Virus from Nigeria, Brazil and Mexico available in the NCBI Nucleotide database was analyzed for genomic analysis and phylogeny. Transmembrane and conserved domains were also analyzed.
Results: The phylogenetic analysis of polyprotein gene showed ancestral genetic diversification, the Nigerian lineage which formed a cluster is evolutionary not related with the Brazilian and Mexican lineages. The Brazilian and Mexican lineages are interrelated forming another cluster from the phylogenetic analysis. Transmembrane domains are found in Nigerian strains but absent in the Brazilian and Mexican strains. The genomic analysis of the conserved domains showed that Nigerian isolates has three conserved domains whereas Brazilian and Mexican has ten to sixteen conserved domains. The three conserved domains (Flavi_glycoprot super family; Flavi_E_C; Flavi_E_stem) found in Nigerian strains were present in the Brazilian and Mexican strains.
Conclusion: This findings strengthen one of the genomic evidences for the outbreak in Brazil and Mexico whereas there is none in Nigeria. This study has also provided genomic epidemiological insights for the circulating strains of Zika virus in these countries.
Keywords: Zika, Virus, Polyprotein gene, Nigeria, Brazil, Mexico, Genome
Corresponding author: Oladipo E.K., Department of Microbiology, Laboratory of Molecular Biology and Bioinformatics, Adeleke University, Ede, Osun State, Nigeria. +2348035369472