Manasik Ali posteradmin
MOLECULAR CHARACTERIZATION OF VIRULENCE GENES OF ESBL-PRODUCING KLEBSIELLA PNEUMONIAE ISOLATED FROM DIFFERENT HOSPITALS IN KHARTOUM STATE, SUDAN
Manasik Ali1*, Hisham N. Altayeb2, Mushal Allam3, Faisal Hammad Mekky Koua4,5
1Department of Microbiology & Molecular Biology, Faculty of Science & Technology, Al Neelain university, El-Baladiya Ave PO Box 12702, Khartoum, Sudan (Expected). 2Microbiology Department, College of Medical Laboratory Sciences, Sudan University of Science and Technology, Khartoum, Sudan. 3Sequencing Core Facility, National Health Laboratory Service, Johannesburg, South Africa.
4National University Research Institute-NURI, National University, Air St., PO Box 3783, Khartoum, Sudan. 5Department of Biochemistry & Molecular Biology, Faculty of Science & Technology, Al Neelain University, El baladiya Ave, PO Box 12702, Khartoum, Sudan.
Background and Aim: Klebsiella pneumoniae is an important Gram-negative rod-shape bacillus often causes range of infections to human. It is ubiquitous to different environments owing its ability to develop specific niche adaptation. The emergence of highly drug-resistant strains is particularly intriguing. K. pneumoniae is a serious public health threatening pathogen. Here, antibiotic resistant genes and virulent factors of circulating ESBL-producing strains isolated from Sudanese patients will be assessed. Methods: The prevalence of the virulence factors and genotypes of CTX-M subgroups, TEM and SHV genes in clinical isolates and their correlation with the degree drug-resistance will be investigated. Thus, antibiotic susceptibility profile, (mrkD, K2, magA, entB and rmpA) and the molecular structures of drug resistant genes (TEM, SHV, CTX-M) of K. pneumoniae isolates will be studied thoroughly to get more insights. Sub-genotypes of ESBLs and virulent factors will be determined by analyzing the whole-genome sequencing by adopting Illumina Next-generation sequencing approach and compare it with K. pneumonie database. Results: We will expect to find different genes of ESBL-producing K. pneumoniae and virulence genes, genomic analysis will identify various antibiotic resistance genes associated with resistance to b-lactams, aminoglycosides, fluoroquinolones, macrolide, lincosamide and streptogramins, rifampicin, sulfonamides, trimethoprim, phenicols and tetracycline and We expect the presence of virulence genes in ESBL-producing K. pneumoniae. Conclusions: Following the outcome of the present study a clear understanding of the relationship between the virulence factors and multi-drug resistance in K. pneumoniae will be expected. Hence, it can be very helpful to manage a strategy to overcome the infections caused by K. pneumoniae.