Omer Osman poster

Omer Osman poster

Abstract Title  Comparative   study between Polymerase Chain Reaction (PCR) and Recombanse Polymerase Amplification (RPA) techniques in diagnosis of pulmonary tuberculosis in Sudan
Author and Affiliations Omer Osman Ali Albadawi B.Sc. (Honours) of Zoology, Faculty of Science

Khartoum University, 2015, M.Sc. in genetics and molecular biology, 2018.

Abstract content This study was performed to assess the sensitivity of Recombinase Polymerase Amplification (RPA) compare to polymerase chain reaction (PCR) in diagnosis of pulmonary tuberculosis in Sudan.

Sputum samples of positive tuberculosis patients were collected from the diagnostic laboratory of Abu Anga Hospital, Omdurman. Sputum samples were subjected to PCR and RPA detecting the insertion sequence (IS) 6110, a region that has been shown to have high sensitivity for diagnosing tuberculosis. Out of 30 sputum samples that were subjected to PCR 14 samples (46.6%) showed positive result and 16 samples (53.4%) were negative. While, 19 samples (63.3%) from that subjected to RPA showed positive result and 11 samples (36.7%) were negative. These results showed that the RPA device is significantly more sensitive than PCR (P < 0.0003). Because it recognizes 19 samples (63.3%) as positive, however PCR recognize just 14 samples (46.6%) as positive.

Conclusion

RPA device is significantly more sensitive than PCR (P < 0.0003). Because it detects 19 samples (63.3%) of microscopy positive samples as positive, however PCR detect just 14 samples (46.6%) as positive.

 

 

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