H. Pylori – Alaaadmin
MOLECULAR CHARACTERIZATION OF HELICOBACTER PYLORI IN PATIENTS WITH UPPER GASTROINTESTINAL DISORDERS, IN WAD MEDANI, GEZIRA STATE, SUDAN 2017_2018
Alaa Faroge Ibrahim, Medical Microbiology Department, faculty of Medical Laboratory Sciences, University of Gezira, (presenter)
Prof. Omer Hassan Arabi, faculty of Animal Production, University of Gezira
Dr. Hisham N- Altayeb Mohamed, College of Medical Laboratory, Sudan University of Science and Technology
This cross sectional study aimed for molecular characterization of H.pylori (H.pylori) from gastric biopsy by 16s rRNA from patients with upper gastrointestinal disorders, in Wad Medani city, Gezira State, Sudan. H.pylori is Gram negative curved rod bacterium, its infection distributed worldwide. It is the major cause of gastritis, duodenitis, peptic ulcer, gastric lymphoma, adenocarcinoma and esopharyngeal cancer. This study include 55 patients, 25 males and 30 females complain from gastric disorder attending for Oesophago-gastro duodenoscopy, their age ranged from 17 to 85 years, from September 2017 to April 2018.Two antrum Gastric biopsy Samples were collected for detection of Helicobacter pylori by conventional Polymerase chain reaction (PCR) using primers target 16s rRNA gene and direct Gram stain. Out of 55 samples 24(43,6%) were positive by PCR, out of 50 samples Gram negative curved bacilli were detected in 20 (40%), and DNA sequencing of amplified PCR products of two samples showed substitution in 16s rRNA gene from (A/C) at position 248380 of reference sequence of Helicobacter pylori strains FDAARGOS_300 USA (Accession number CP027404.1). This study concluded that the H. pylori could possibly be considered one of the main causes of gastritis and Duodenitis, also direct detection of H. pylori by Gram stain statistically not differ from PCR.