ASSESSMENT OF CYTOCHROME P450

ASSESSMENT OF CYTOCHROME P450

ASSESSMENT OF CYTOCHROME P450 2E1 (G-1293C, RS3813867) GENE POLYMORPHISM IN SUDANESE PATIENTS WITH ACUTE MYELOID LEUKAEMIA & controls

 

Alaa. M. Hamad1*, Hanan. B. Eltahir2, Faisal M. Fadlelmola3,4

 

1*Department of Biotechnology, School of pharmacy, Ahfad university for women, Sudan.

2Department of Biochemistry, Faculty of Medicine, University of El Imam El Mahdi, Sudan.

3Centre for Bioinformatics & Systems Biology, Faculty of Science, University of Khartoum, Sudan.

4H3ABioNet University of Khartoum Node as members of the H3Africa Consortium.

Corresponding author Email: alaahamad607@gmail.com*

 

Background Acute myeloid leukemia (AML) is a complicated, heterogeneous disease involving the presence of a clonal expansion of neoplastic myeloid cells with variable degrees of differentiation and varying clinical, morphologic, immunologic, molecular characteristics and cytogenetic abnormalities. The exact cause of leukemia is unknown. A combination of genetic factors and environmental factors are believed to play a role, an exposure to xenobiotic like cigarette smoking and other chemicals was found to be associated with polymorphism of cytochrome 2E1 in Eastern countries like Chinese and Japanese patients with AML, but in Africa was found to be markedly different. The CYP2E1 gene activates nitrosamine chemicals into more toxic or carcinogenic forms, therefore it receives a great deal of attention in terms of occupational cancers.

 The aim of this study was to investigate the role of polymorphism in cytochrome 2E1 G-1293C (rs3813867) in developing AML in Khartoum state. After taken then informed consent, 25 AML patients and 38 healthy controls were included in the study.

Methods DNA was isolated from whole blood. Genotyping of 2E1 G-1293C (rs3813867) was performed by PCR-RFLP analysis followed by DNA sequencing for the flanking region of the gene.  

Results The frequency of the c1 allele of CYP2E1 gene was found to be the predominant one in both patients and healthy controls 100% (p=0.02) with c1/c1 genotype.

Conclusion Our results suggest that CYP2E1*5B variant rs3813867 plays no role in development of AML in Sudanese samples. 

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