obesity in Sudanese childrenadmin
Association of a common rs9939609 variant in the fat mass and obesity-associated (FTO) gene with obesity in Sudanese children
1 Alyaa Awad 1, Azza Ahmed, Faisal M. Fadlelmola 1
1 Centre for Bioinformatics & Systems Biology (CBSB), Faculty of Science, University of
Background: Obesity disease develops as a result of interactions between the individual’s genetic component and his exposure to environmental risk factors such as lifestyle and food habits. It is well known that the Fat mass and obesity associated gene (FTO) is responsible of the body mass index (BMI) and the risk of obesity. Aims: The study objective was to assess the association of FTO gene rs9939609 polymorphism with BMI in a sample of Sudanese children.
Methods: Twenty-seven subjects with ages ranging between 7-14 years were sampled from different locations of Khartoum State during the period of January to March 2016. Anthropometric measurements were taken from the subjects and then they were categorized into cases and controls based on their age, gender and BMI. Buccal swab samples were collected and the genomic DNA was extracted using guanidine chloride extraction method. PCR was performed using FTO rs9939609 forward and reverse primers. PCR-RFLP was performed using the Sca1 restriction enzyme.
Results: Out of twenty-seven samples, eleven participants show the homozygous wild type TT genotype with 41%, five participants show heterozygous TA genotype with 18% and eleven participants show homozygous mutant AA genotype with 41%. The results showed that the polymorphic A allele had the highest risk of obesity with p-value <0.0001.
Conclusions: The study showed that there is a strong association between FTO rs9939609 and the risk of obesity among the studied sample of Sudanese children in Khartoum State. Further studies are required to confirm these findings.