MICRO-RNA 153 EXPRESSIONS IN BREAST CANCER IN SUDAN

MICRO-RNA 153 EXPRESSIONS IN BREAST CANCER IN SUDAN

 

      MICRO-RNA 153 EXPRESSIONS IN BREAST CANCER IN SUDAN

 A POTENTIAL BIOMARKER?

 

   Duaa Ali Ahmed1, 2; Mohammed.O.Elseideg 2;  Tamador Elhadi 2; Hiba Salah 2; Mohamed.A.wadatallah3

   Muntaser E Ibrahim 2

 

 1 Department of Internal Medicine, Sudan Medical Specialization Board.

 2 Department of Molecular Biology, Institute of Endemic Diseases, University of Khartoum, Khartoum,                        

   Sudan

 3 Ibrahem Malik teaching hospital, University of Khartoum.

 

BACKGROUND: Breast cancer is the most common cancer in women worldwide constituting 24.2% of newly diagnosed cancer in women. In Sudan it’s a major health problem with incidence rate 25.1/100,000. Major challenge in breast cancer management is searching for sensitive, specific and minimally invasive biomarkers for early detection of breast cancer. Micro-RNA(miR) are a class of small noncoding RNA that regulate gene expression at post-transcriptional level. MiR plays important roles in tumor initiation, metastasis and drug resistance. Wide genome Methylome analysis of breast cancer in Sudanese revealed differential methylation of miRNA 153-2 in breast cancer with high specificity.

AIM: The primary aim of this study is to investigate whether miR153 expression is altered in breast cancer tissue compared with healthy control in Sudanese patients.

Methodology: This is an observational, pilot, case control study. Seven confirmed breast cancer patients were included. Cancerous breast tissues (cases) were taken during surgery and a (control) samples were taken from healthy margins from the same patients. Easy blue was used for RNA extraction and expression was measured by Real time PCR, the 2nd most sensitive and specific approach for RNA detection after next generation sequencing.

Results:  miRNA-153 is found to be differentially overexpressed by 39.4 fold more -on average- in breast cancer tissues compared to normal controls.  This outcome, in case of further validation in a larger sample, may have an important diagnostic and prognostic role.

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