TELOMERE VARIATION & BREAST CANCER AND HCC

TELOMERE VARIATION & BREAST CANCER AND HCC

THE ROLE OF TELOMERE COPY NUMBER VARIATION IN PROGNOSIS OF BREAST CANCER AND HEPATOCELLULAR CARCINOMA IN SUDANESE PATIENTS, A CASE-CONTROL STUDY.

Maram A. Suliman1,2, Muntasir E. Ibrahim2

1 Department of Human Biology, Ibn Sina University, Khartoum, Sudan.

2 Department of molecular Biology, Institute of Endemic Diseases, University of Khartoum, Khartoum, Sudan.

1 maram.a.suliman@gmail.com

2 mibrahim@iend.org

ABSTRACT

Aim: To assess the role of Telomere Length (TL) variance in Breast Cancer (BC) and Hepatocellular Carcinoma (HCC) pathology in Sudan and to verify if TL can be a reliable early detection marker for both cancers using Real Time PCR.

Background: Previously, telomere length was recommended as clinical outcome predictor in different cancers.

Methods: In this study, genomic DNA was extracted from 58 tumor and paired non-tumorous tissues samples plus blood samples from BC patients and their age matched healthy controls, also 42 blood samples were used from HCC patients and their age matched healthy controls. A real-time PCR assay was used to estimate genomic telomeres copy numbers and thus their length.

Results: Using the 2 ∆∆Ct method, our results showed that there was an increase in the average of copies in tumor tissues of BC cases (71.4 copies) in compare to cases’ normal tissues (19.3 copies), and (10.9 copies) in cases blood in compare to (14.8 copies) the blood of their matched healthy controls. Consistently TL was long in (63%) of BC patients, unlike in HCC patients where only (9.5%) had long telomeres where HCC samples exhibited very few telomeric DNA copies (0.47 copies) in cases and (0.2 copies) in their matched healthy controls. TL was found to be greatly affected by a female pregnancy status in BC patients (P = 0.02). Also the age at which a female get pregnant for the first time showed a strong effect on TL (P = 0.02). In HCC patients, TL showed a very strong positive correlation with age (P =0.01), although not statistically significant in BC patients, possibly due to the low number of this population. Another strong cofounder that found to affect the TL was Ethnicity were it showed significant difference in BC (P = 0.012) and HCC (P = 0.035).

Conclusion: In light of the above results, Telomeric copy number can be an early predictor for Breast Cancer and Hepatocellular Carcinoma as well.

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