HL & ALCLadmin
GENE EXPRESSION PROFILES OF HODGKIN LYMPHOMA (HL) And ANAPLASTIC LARGE CELL LYMPHOMA (ALCL) CELL LINES
Nihad Hassan1 , Kamal H. Mohamed 2 , Diponkar Banerjee 3, Faisal M. Fadlelmola 1
1 Centre for Bioinformatics and Systems Biology, Faculty of Science, University of Khartoum, Sudan
2 Radiation and Isotope Centre of Khartoum (RICK)
3 Division of Anatomical Pathology, Eastern Ontario Regional Laboratory Association and The Ottawa Hospital
Background: Hodgkin’s lymphoma (HL) and Anaplastic large cell lymphoma (ALCL) are subtypes of human malignant lymphomas. The borderline is not well characterised in the context of morphologic, immunophenotypic, and clinical characteristics between the two diseases, as well as they both express CD30. The use of gene expression microarray in diseases is a genomic technology that improves and provides more accurate diagnosis. Aim and Method: Here TM4 suits software was used to analyse gene expression microarray data for four haematopoietic cell lines comprised KMH2 and L428 of human HL derived cell lines, and DEL and SR786 of ALCL-derived ones. Results: An overall of hundred and twenty-one genes were showed to have a significant differential expressed patterns in all cell lines, among which five of them were considered to be a diagnostic biomarker candidate. Furthermore, the candidate differentially expressed genes were subjected to pathway and protein-protein interactions analysis. The study showed that three of them were found to be involved in different 83 pathways and the majority were shown to have interactions with many other proteins. Conclusion: Significant findings have showed a resemblance to other symmetrical studies in the literature. In contrast some genes have exhibited different levels of expression than has been reported. On the other hand, part of the findings has not been previously reported in Lymphoma literature but it has been involved in other types of cancer. Further downstream analysis with tissue microarray and protein array is needed for the validation and confirmation of these results.