METHYLOTROPHIC BACTERIAL ISOLATES

METHYLOTROPHIC BACTERIAL ISOLATES

PROTEOMICS AND METABOLOMICS PROFILING OF PINK-PIGMENTED FACULTATIVE METHYLOTROPHIC BACTERIAL ISOLATES

Tayseer Qasim1* and Faisal Hammad Mekky Koua1,2*

1Department of Biochemistry & Molecular Biology, Faculty of Science & Technology, Al Neelain University, El-Baladiya Ave, PO Box 12702, Khartoum, Sudan. 2National University Research Institute-NURI, National University, Air St, PO Box 3783, Khartoum, Sudan.

*Email: Tyseer114@hotmail.com

Haram Mohammed2 ,Department of Biology & Biotechnology Faculty of Science & Technology Al Neelain University ,El Baladiya Ave  PO Box 12702 Khartoum Sudan.

Tipyan Omer Mahgoub3, Department of Biology & Biotechnology Faculty of Science & Technology Al Neelain University, El Baladiya Ave  PO Box 12702 Khartoum Sudan.

Background and aim: pink-pigmented facultative methylotrophic (PPFM) bacteria are a group of heterotrophs that are characterized by their ability to metabolize C1 compound, and to the distinctive pigmentation owing to the carotenoids rendering them to be tolerant to radiation and high light intensities. Most methylotrophs are beneficial to plant and human health, however, some have been reported to be pathogen to human. Nonetheless, little is known about the mechanisms of these methylotrophs with their environments as well as their responses to the different biotic and abiotic stresses in terms of secreted peptides/proteins and metabolites, i.e. secretome and metabolome, respectively. Methods: in the present study, nine cultivable water methylotrophic isolates from the Nile river; G1 (A12, A11, B32, B12N), G2 (A11N, B31, A12N), G3 (A22) and G4 (B11N), were identified using conventional microbiological methods. Comprehensive phylogenetic analysis to the mxaF and xoxF genes of the methanol dehydrogenase as well as the 16S rDNA gene were used in comparison with 30 reported PPFM. Results: G1, G3, G3 and G4 were successfully identified as Moraxella kingii, Cardiobacterium hominis, Pasteurella haemolytica type T, p. trehalosi and Pasteurella haemolytica type T, p. haemolytica, respectively, and further tested for their resistance to antibiotics. 16S rDNA sequencing as well as a total genome sequencing using Illumina next-generation sequencing will be considered. The response of these strains to antibiotics, inter-species interactions with other microorganism and heavy metals will be evaluated focusing on the secretome and extracellular metabolome. Conclusion: all together, the present study will provide novel information regarding methylotrophy and the role of abiotic stresses in enhancing or diminishing the methylotrophic metabolism in these PPFMs.

 

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